Coding
Part:BBa_K4202008:Design
Designed by: Hongying LIN Group: iGEM22_ZJU-China (2022-09-29)
Amorphous Calcium Carbonate Binding Protein(ACCBP)(with signal peptide)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 189
Illegal AgeI site found at 452 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 658
Design Notes
We are not sure whether the protein will be scecreted extracellularly with signal sequence.Therefore, we designed two parts, one with signal sequence, and another without the signal sequence.BBa_K4202008 is the one with signal sequence
Source
The sequence of this part was originally retrieved from GenBankDQ473430.1. The codons of this sequence was optimized by Genscipt.